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rnf8 interactions  (Proteintech)


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    Structured Review

    Proteintech rnf8 interactions
    a , Schematic of sensitized UBAL degradation CRISPR-Cas9 screen. b , Gene effects and gene scores calculated for individual genes analyzed in the targeted degradation screen. c , STRING network map of the identified genes with a 10% FDR cutoff, where each bubble color represents an enriched GO term functional cluster. d , Fluorescence histograms of FSP1 KO cells expressing FSP1-GFP in the indicated cell lines. e , Quantification of median fluorescence intensity (MFI) change in GFP from ( d ). f , Immunoblot of lysates from ( d ). g-h , Kinetics of GFP fluorescence decay of FSP1 KO cells expressing FSP1-GFP in control or RFK KO cells with or without the loss of <t>RNF8</t> ( g ) or RFK/RNF8 DKO (double knock-out) cells expressing the indicated RNF8 variants ( h ) following doxycycline washout. i , FSP1-GFP or RNF8 S-tag immunoprecipitation from FSP1 KO cells expressing FSP1-GFP in the indicated cell lines and blotting for RNF8 (top) or FSP1 (middle). j , Model illustrating the mechanism by which vitamin B2 metabolism and FAD synthesis impacts FSP1 activity and stability to prevent ferroptosis.
    Rnf8 Interactions, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnf8 interactions/product/Proteintech
    Average 93 stars, based on 24 article reviews
    rnf8 interactions - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis"

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis

    Journal: bioRxiv

    doi: 10.1101/2025.08.05.668752

    a , Schematic of sensitized UBAL degradation CRISPR-Cas9 screen. b , Gene effects and gene scores calculated for individual genes analyzed in the targeted degradation screen. c , STRING network map of the identified genes with a 10% FDR cutoff, where each bubble color represents an enriched GO term functional cluster. d , Fluorescence histograms of FSP1 KO cells expressing FSP1-GFP in the indicated cell lines. e , Quantification of median fluorescence intensity (MFI) change in GFP from ( d ). f , Immunoblot of lysates from ( d ). g-h , Kinetics of GFP fluorescence decay of FSP1 KO cells expressing FSP1-GFP in control or RFK KO cells with or without the loss of RNF8 ( g ) or RFK/RNF8 DKO (double knock-out) cells expressing the indicated RNF8 variants ( h ) following doxycycline washout. i , FSP1-GFP or RNF8 S-tag immunoprecipitation from FSP1 KO cells expressing FSP1-GFP in the indicated cell lines and blotting for RNF8 (top) or FSP1 (middle). j , Model illustrating the mechanism by which vitamin B2 metabolism and FAD synthesis impacts FSP1 activity and stability to prevent ferroptosis.
    Figure Legend Snippet: a , Schematic of sensitized UBAL degradation CRISPR-Cas9 screen. b , Gene effects and gene scores calculated for individual genes analyzed in the targeted degradation screen. c , STRING network map of the identified genes with a 10% FDR cutoff, where each bubble color represents an enriched GO term functional cluster. d , Fluorescence histograms of FSP1 KO cells expressing FSP1-GFP in the indicated cell lines. e , Quantification of median fluorescence intensity (MFI) change in GFP from ( d ). f , Immunoblot of lysates from ( d ). g-h , Kinetics of GFP fluorescence decay of FSP1 KO cells expressing FSP1-GFP in control or RFK KO cells with or without the loss of RNF8 ( g ) or RFK/RNF8 DKO (double knock-out) cells expressing the indicated RNF8 variants ( h ) following doxycycline washout. i , FSP1-GFP or RNF8 S-tag immunoprecipitation from FSP1 KO cells expressing FSP1-GFP in the indicated cell lines and blotting for RNF8 (top) or FSP1 (middle). j , Model illustrating the mechanism by which vitamin B2 metabolism and FAD synthesis impacts FSP1 activity and stability to prevent ferroptosis.

    Techniques Used: CRISPR, Functional Assay, Fluorescence, Expressing, Western Blot, Control, Knock-Out, Immunoprecipitation, Activity Assay



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    rnf8 interactions  (Proteintech)
    93
    Proteintech rnf8 interactions
    a , Schematic of sensitized UBAL degradation CRISPR-Cas9 screen. b , Gene effects and gene scores calculated for individual genes analyzed in the targeted degradation screen. c , STRING network map of the identified genes with a 10% FDR cutoff, where each bubble color represents an enriched GO term functional cluster. d , Fluorescence histograms of FSP1 KO cells expressing FSP1-GFP in the indicated cell lines. e , Quantification of median fluorescence intensity (MFI) change in GFP from ( d ). f , Immunoblot of lysates from ( d ). g-h , Kinetics of GFP fluorescence decay of FSP1 KO cells expressing FSP1-GFP in control or RFK KO cells with or without the loss of <t>RNF8</t> ( g ) or RFK/RNF8 DKO (double knock-out) cells expressing the indicated RNF8 variants ( h ) following doxycycline washout. i , FSP1-GFP or RNF8 S-tag immunoprecipitation from FSP1 KO cells expressing FSP1-GFP in the indicated cell lines and blotting for RNF8 (top) or FSP1 (middle). j , Model illustrating the mechanism by which vitamin B2 metabolism and FAD synthesis impacts FSP1 activity and stability to prevent ferroptosis.
    Rnf8 Interactions, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnf8 interactions/product/Proteintech
    Average 93 stars, based on 1 article reviews
    rnf8 interactions - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    crystal structure of the rnf8-ubc13 interaction  (Helmholtz Zentrum fur Infektionsforschung GmbH)
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    Helmholtz Zentrum fur Infektionsforschung GmbH crystal structure of the rnf8-ubc13 interaction
    a , Schematic of sensitized UBAL degradation CRISPR-Cas9 screen. b , Gene effects and gene scores calculated for individual genes analyzed in the targeted degradation screen. c , STRING network map of the identified genes with a 10% FDR cutoff, where each bubble color represents an enriched GO term functional cluster. d , Fluorescence histograms of FSP1 KO cells expressing FSP1-GFP in the indicated cell lines. e , Quantification of median fluorescence intensity (MFI) change in GFP from ( d ). f , Immunoblot of lysates from ( d ). g-h , Kinetics of GFP fluorescence decay of FSP1 KO cells expressing FSP1-GFP in control or RFK KO cells with or without the loss of <t>RNF8</t> ( g ) or RFK/RNF8 DKO (double knock-out) cells expressing the indicated RNF8 variants ( h ) following doxycycline washout. i , FSP1-GFP or RNF8 S-tag immunoprecipitation from FSP1 KO cells expressing FSP1-GFP in the indicated cell lines and blotting for RNF8 (top) or FSP1 (middle). j , Model illustrating the mechanism by which vitamin B2 metabolism and FAD synthesis impacts FSP1 activity and stability to prevent ferroptosis.
    Crystal Structure Of The Rnf8 Ubc13 Interaction, supplied by Helmholtz Zentrum fur Infektionsforschung GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/crystal structure of the rnf8-ubc13 interaction/product/Helmholtz Zentrum fur Infektionsforschung GmbH
    Average 90 stars, based on 1 article reviews
    crystal structure of the rnf8-ubc13 interaction - by Bioz Stars, 2026-03
    90/100 stars
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    Image Search Results


    a , Schematic of sensitized UBAL degradation CRISPR-Cas9 screen. b , Gene effects and gene scores calculated for individual genes analyzed in the targeted degradation screen. c , STRING network map of the identified genes with a 10% FDR cutoff, where each bubble color represents an enriched GO term functional cluster. d , Fluorescence histograms of FSP1 KO cells expressing FSP1-GFP in the indicated cell lines. e , Quantification of median fluorescence intensity (MFI) change in GFP from ( d ). f , Immunoblot of lysates from ( d ). g-h , Kinetics of GFP fluorescence decay of FSP1 KO cells expressing FSP1-GFP in control or RFK KO cells with or without the loss of RNF8 ( g ) or RFK/RNF8 DKO (double knock-out) cells expressing the indicated RNF8 variants ( h ) following doxycycline washout. i , FSP1-GFP or RNF8 S-tag immunoprecipitation from FSP1 KO cells expressing FSP1-GFP in the indicated cell lines and blotting for RNF8 (top) or FSP1 (middle). j , Model illustrating the mechanism by which vitamin B2 metabolism and FAD synthesis impacts FSP1 activity and stability to prevent ferroptosis.

    Journal: bioRxiv

    Article Title: Vitamin B2 metabolism promotes FSP1 stability to prevent ferroptosis

    doi: 10.1101/2025.08.05.668752

    Figure Lengend Snippet: a , Schematic of sensitized UBAL degradation CRISPR-Cas9 screen. b , Gene effects and gene scores calculated for individual genes analyzed in the targeted degradation screen. c , STRING network map of the identified genes with a 10% FDR cutoff, where each bubble color represents an enriched GO term functional cluster. d , Fluorescence histograms of FSP1 KO cells expressing FSP1-GFP in the indicated cell lines. e , Quantification of median fluorescence intensity (MFI) change in GFP from ( d ). f , Immunoblot of lysates from ( d ). g-h , Kinetics of GFP fluorescence decay of FSP1 KO cells expressing FSP1-GFP in control or RFK KO cells with or without the loss of RNF8 ( g ) or RFK/RNF8 DKO (double knock-out) cells expressing the indicated RNF8 variants ( h ) following doxycycline washout. i , FSP1-GFP or RNF8 S-tag immunoprecipitation from FSP1 KO cells expressing FSP1-GFP in the indicated cell lines and blotting for RNF8 (top) or FSP1 (middle). j , Model illustrating the mechanism by which vitamin B2 metabolism and FAD synthesis impacts FSP1 activity and stability to prevent ferroptosis.

    Article Snippet: For immunoprecipitation of FSP1 and RNF8 interactions, 1 mg of pre-cleared lysates were incubated with 10 μL of ChromoTek GFP-Trap Magnetic Agarose or 10 μL of S-protein Agarose (Millipore Sigma, no. 69704) for 1 h at 4°C with end-over-end rotation.

    Techniques: CRISPR, Functional Assay, Fluorescence, Expressing, Western Blot, Control, Knock-Out, Immunoprecipitation, Activity Assay